Mix-and-read bioluminescent copper detection platform using a caged coelenterazine analogue-Reference-Cited by-同舟云学术

Mix-and-read bioluminescent copper detection platform using a caged coelenterazine analogue

Published:2021 Issue:20 Volume:146 Page:6139-6144
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Nishihara Ryo¹²³^ORCID,Kurita Ryoji¹²⁴^ORCID

Affiliation:

1. National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

2. DAILAB, DBT-AIST International Centre for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science and Technology (AIST), Central 5-41, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan

3. Japan Science and Technology Agency (JST), PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

4. Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan

Abstract

Bioluminescence (BL) detection of copper in human serum was achieved by developing a caged luciferin. The luciferin reacts only with copper and exhibits luminescence with human serum albumin, which provides a “luciferase-independent” BL copper assay.

Funder

Japan Society for the Promotion of Science

Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering

Research Foundation for Opto-Science and Technology

Precursory Research for Embryonic Science and Technology

Japan Agency for Medical Research and Development

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN01292D