Analysis of intracellular α-keto acids by HPLC with fluorescence detection-Reference-Cited by-同舟云学术

Analysis of intracellular α-keto acids by HPLC with fluorescence detection

Published:2020 Issue:20 Volume:12 Page:2555-2559
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Fujiwara Takuya¹²³⁴,Hattori Ayuna⁵⁶⁷⁴,Ito Takahiro⁸⁹¹⁰⁴¹¹,Funatsu Takashi¹²³⁴,Tsunoda Makoto¹²³⁴^ORCID

Affiliation:

1. Graduate School of Pharmaceutical Sciences

2. University of Tokyo

3. Tokyo 113-0033

4. Japan

5. Division of Hematological Malignancy

6. National Cancer Center Research Institute

7. Tokyo 104-0045

8. Institute for Frontier Life and Medical Sciences

9. Kyoto University

10. Kyoto 606-8507

11. Department of Biochemistry & Molecular Biology

Abstract

We developed a method to analyze six α-keto acids in K562 cells by HPLC with fluorescence detection, using 1,2-diamino-4,5-methylenedioxybenzene as a derivatization reagent.

Funder

American Cancer Society

Center of Innovation Program

Japan Agency for Medical Research and Development

Daiichi Sankyo Foundation of Life Science

Japan Society for the Promotion of Science

Yasuda Memorial Medical Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AY/D0AY00556H

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