Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes-Reference-Cited by-同舟云学术

Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes

Published:2022 Issue:17 Volume:22 Page:3172-3186
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Compera Nina¹,Atwell Scott¹,Wirth Johannes¹^ORCID,von Törne Christine²^ORCID,Hauck Stefanie M.²,Meier Matthias¹³^ORCID

Affiliation:

1. Helmholtz Pioneer Campus, Helmholtz Zentrum München, Munich, Germany

2. Metabolomics and Proteomics Core, Helmholtz Zentrum München, Munich, Germany

3. TUM School of Medicine, Technical University of Munich, Munich, Germany

Abstract

In this study, we developed a microfluidic large-scale integration chip platform to automate longitudinal 3D cell culture studies. Proteome analysis of on chip cultured adipocytes under changing glucose conditions revealed metabolic stress profiles.

Funder

H2020 European Research Council

Helmholtz Association

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00245K

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