Compartmentalized microfluidic chambers enable long-term maintenance and communication between human pluripotent stem cell-derived forebrain and midbrain neurons-Reference-Cited by-同舟云学术

Compartmentalized microfluidic chambers enable long-term maintenance and communication between human pluripotent stem cell-derived forebrain and midbrain neurons

Published:2021 Issue:20 Volume:21 Page:4016-4030
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Tong Ziqiu¹^ORCID,Kwak Eunbi¹,Aguiar Alita¹,Peng Bo²,Pouton Colin W.¹,Voelcker Nicolas H.¹²³^ORCID,Haynes John M.⁴

Affiliation:

1. Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria, 3052, Australia

2. Commonwealth Scientific and Industrial Research Organisation (CSIRO), Clayton, Victoria, 3168, Australia

3. Melbourne Centre for Nanofabrication, Victorian Node of the Australian National Fabrication Facility, Clayton, Victoria, 3168, Australia

4. Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria, 3052, Australia

Abstract

Open coculture microfluidic chambers embedding Matrigel for 3D culture of hPSCderived forebrain and midbrain neurons over 5 months with functional neural activity.

Funder

Australian Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00505G

Reference59 articles.

1. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling

2. Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

3. Pharmaceutical applications of embryonic stem cells