Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples-Reference-Cited by-同舟云学术

Simultaneous extraction, separation and purification of microbial genomic DNA and total RNA from acidic habitat samples

Published:2015 Issue:3 Volume:7 Page:909-917
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Xie Jianping¹²³⁴⁵,Yun Hui¹²³⁴⁶,Dong Haigang⁷⁸⁴⁹⁸,Zhao Wenya¹²³⁴¹⁰,Wang Guohua¹²³⁴¹⁰,Qiu Guanzhou¹²³⁴¹⁰,Liu Xinxing¹²³⁴¹⁰

Affiliation:

1. School of Mineral Processing and Bioengineering

2. Central South University

3. Changsha

4. China

5. State Key Laboratory of Powder Metallurgy

6. Research Centre for Eco-Environmental Sciences

7. Kunming Institute of Precious Metals

8. Kunming

9. State Key Laboratory of Advanced Technologies for Comprehensive Utilisation of Platinum Metals

10. Key Laboratory of Biometallurgy

Abstract

DNA and RNA simultaneously extracted fromA. fusing the optimised method. (a) Total nucleic acid extracted fromA. f: lane M1, 1 kb ladder; lane M2,HindIII-cut lambda molecular size marker; lanes 1–4, biological replicates. (b) DNA precipitated by isopropanol. (c) RNA precipitated by LiCl.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AY/C4AY01608D

Reference50 articles.

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4. Effects of Acid Mine Drainage on the Estuarine Food Web, Britannia Beach, Howe Sound, British Columbia, Canada

5. Negative pH, efflorescent mineralogy, and consequences for environmental restoration at the Iron Mountain Superfund site, California

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