Enhancing the sequence coverage of nanodiamond-extracted early secretory proteins from the <i>Mycobacterium tuberculosis</i> complex-Reference-Cited by-同舟云学术

Enhancing the sequence coverage of nanodiamond-extracted early secretory proteins from the Mycobacterium tuberculosis complex

Published:2024 Issue:22 Volume:16 Page:3464-3474
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Soo Po-Chi¹,Lee Ching-Chieh²,Shie Meng-Fu²,Patil Avinash A.²,Descanzo Mhikee Janella N.²,Chin Ya-Ching²,Chen Hsi-An²,Horng Yu-Tze¹,Lin Chih-Bin³,Lee Jen-Jyh³,Chiang Cheng-Kang⁴^ORCID,Peng Wen-Ping²^ORCID

Affiliation:

1. Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan

2. Department of Physics, National Dong Hwa University, Shoufeng, Hualien, Taiwan

3. Department of Internal Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan

4. Department of Chemistry, National Dong Hwa University, Shoufeng, Hualien, Taiwan

Abstract

The sequence coverage of CFP-10 and ESAT-6 proteins from the Mycobacterium tuberculosis complex (MTC) has been greatly enhanced >94% with the Cl-CCA matrix and eliminating interferences from bovine serum albumin and α-crystallin proteins.

Funder

Ministry of Science and Technology, Taiwan

National Dong Hwa University

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AY/D4AY00314D

Reference42 articles.

1. 100% protein sequence coverage: a modern form of surrealism in proteomics

2. Comprehensive Middle-Down Mass Spectrometry Characterization of an Antibody–Drug Conjugate by Combined Ion Activation Methods

3. Extensive Characterization of Heavily Modified Histone Tails by 193 nm Ultraviolet Photodissociation Mass Spectrometry via a Middle–Down Strategy

4. Toward an Optimized Workflow for Middle-Down Proteomics

5. Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques