BSA blocking in enzyme-linked immunosorbent assays is a non-mandatory step: a perspective study on mechanism of BSA blocking in common ELISA protocols-Reference-Cited by-同舟云学术

BSA blocking in enzyme-linked immunosorbent assays is a non-mandatory step: a perspective study on mechanism of BSA blocking in common ELISA protocols

Published:2015 Issue:121 Volume:5 Page:100077-100083
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Ahirwar Rajesh¹²³⁴²,Bariar Shilpi¹²³,Balakrishnan Abitha¹²³,Nahar Pradip¹²³⁴²

Affiliation:

1. CSIR-Institute of Genomics and Integrative Biology

2. Delhi

3. India-110007

4. Academy of Scientific and Innovative Research

Abstract

Interestingly, in majority of cases, the BSA-blocking and non BSA-blocking procedures produces similar ELISA outcomes.

Funder

Council of Scientific and Industrial Research

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA20750A

Reference21 articles.

1. B. E. Batteiger , Blocking of Immunoblots, Handbook of Immunoblotting of Proteins, ed. O. J. Bjerrum and N. H. H. Heegaard, CRC Press Inc., Boca Raton, Florida, 1988, vol. I, pp. 145–150

2. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G

3. Enzyme‐Linked Immunosorbent Assays

4. A blocking agent and a blocking step are not needed in ELISA, immunostaining dot-blots and Western blots

Cited by 41 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Paper-based immunosensor integrated with bioinspired Cu-polydopamine nanozyme for voltammetric detection of CA-15-3 tumor marker;Sensors and Actuators Reports;2024-12

2. The Severity of Gastrointestinal Disorders and Autistic-Like Behaviors Could Be Associated with a Selective Humoral Response to Bovine Milk Caseins: A Case Series;Gastroenterology Insights;2024-06-26

3. First Demonstration of GaN HEMT-Based Handheld System for Noninvasive Detection of Traumatic Brain Injury Using Saliva;IEEE Sensors Journal;2024-02-01

4. iSERS: from nanotag design to protein assays and ex vivo imaging;Chemical Society Reviews;2024

5. Simplified PCR-Based Quantification of Proteins with DNA Aptamers and Methylcellulose as a Blocking Agent;International Journal of Molecular Sciences;2023-12-26