Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site-Reference-Cited by-同舟云学术

Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site

Published:2019 Issue:48 Volume:55 Page:6934-6937
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Chang Fu¹²³⁴⁵,Sun Yueying¹²³⁴⁵,Yang Dandan¹²³⁴⁵,Yang Wenjuan⁶⁷⁸,Sun Yuanyuan¹²³⁴⁵,Liu Chenghui¹²³⁴⁵^ORCID,Li Zhengping¹²³⁴⁵^ORCID

Affiliation:

1. Key Laboratory of Applied Surface and Colloid Chemistry

2. Ministry of Education

3. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province

4. School of Chemistry and Chemical Engineering

5. Shaanxi Normal University

6. Department of Ultrasound

7. Xijing Hospital Affiliated to The Fourth Military Medical University

8. P. R. China

Abstract

A chimeric DNA-aided RNase H-EXPAR approach is designed for the accurate and specific detection of RNA mutation at single-base resolution.

Funder

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2019/CC/C9CC02700A

Reference28 articles.

1. An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

2. Quantitative real-time RT-PCR – a perspective

3. BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles

4. In situ detection and genotyping of individual mRNA molecules

5. Highly specific imaging of mRNA in single cells by target RNA-initiated rolling circle amplification

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Elimination of Unspecific Amplification in the Exponential Amplification Reaction Using Polyethylene Glycol 200 (PEG 200) as a Cosolute;Analytical Letters;2024-03-13

2. EXPAR for biosensing: recent developments and applications;The Analyst;2024

3. Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay;PLOS ONE;2023-08-22

4. Bacterial drug-resistance and viability phenotyping upon disinfectant exposure revealed by single-nucleotide resolved-allele specific isothermal RNA amplification;Journal of Hazardous Materials;2023-04

5. One-step isothermal amplification strategy for microRNA specific and ultrasensitive detection based on nicking-assisted entropy-driven DNA circuit triggered exponential amplification reaction;Analytica Chimica Acta;2022-04