Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay

Author:

Uhd Jesper1234,Miotke Laura56789,Ji Hanlee P.56789,Dunaeva Marina1011121314,Pruijn Ger J. M.1011121314,Jørgensen Christian Damsgaard1516174,Kristoffersen Emil Laust1819204,Birkedal Victoria1819204ORCID,Yde Christina Westmose212223244,Nielsen Finn Cilius212223244,Hansen Jonas123425,Astakhova Kira1234ORCID

Affiliation:

1. Department of Chemistry

2. Technical University of Denmark

3. 2800 Kgs. Lyngby

4. Denmark

5. Division of Oncology

6. Department of Medicine

7. Stanford University

8. Stanford

9. USA

10. Department of Biomolecular Chemistry

11. Institute for Molecules and Materials

12. Radboud University

13. 6525 GA Nijmegen

14. The Netherlands

15. Department of Mathematics and Computer Science

16. University of Southern Denmark

17. 5230 Odense

18. Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry

19. Aarhus University

20. 8000 Aarhus C

21. Center for Genomic Medicine

22. 4113 Rigshospitalet

23. Copenhagen University Hospital

24. DK-2100 Copenhagen

25. Institute of Molecular Medicine

Abstract

Fast and reliable assay for amplification-free absolute quantification of DNA and RNA in biofluids.

Funder

Villum Fonden

Novo Nordisk Fonden

Arthritis Society

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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