Exploring the entropy-driven amplification reaction and trans-cleavage activity of CRISPR-Cas12a for the development of an electrochemiluminescence biosensor for the detection of the SARS-CoV-2 RdRp gene in real samples and environmental surveillance-Reference-Cited by-同舟云学术

Exploring the entropy-driven amplification reaction and trans-cleavage activity of CRISPR-Cas12a for the development of an electrochemiluminescence biosensor for the detection of the SARS-CoV-2 RdRp gene in real samples and environmental surveillance

Published:2022 Issue:1 Volume:9 Page:162-172
ISSN:2051-8153
Container-title:Environmental Science: Nano
language:en
Short-container-title:Environ. Sci.: Nano

Author:

Zhang Kai¹^ORCID,Fan Zhenqiang¹^ORCID,Ding Yuedi¹,Zhu Sha²,Xie Minhao¹^ORCID,Hao Nan³

Affiliation:

1. NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China

2. Department of Oncology, The Affiliated Wuxi No.2 People's Hospital of Nanjing Medical University, Wuxi, 214000, PR China

3. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, PR China

Abstract

An ECL biosensor based on entropy-driven amplification and CRISPR-Cas12a shear for the detection of the SARS-CoV-2 RdRp gene in environmental surveillance was reported.

Funder

National Natural Science Foundation of China

Government of Jiangsu Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Environmental Science,Materials Science (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/EN/D1EN00645B

Reference61 articles.

1. Ultrasensitive photoelectrochemical microRNA biosensor based on doxorubicin sensitized graphitic carbon nitride assisted by a target-activated enzyme-free DNA walker

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