Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review

Author:

Miles A. J.1234ORCID,Janes Robert W.5674ORCID,Wallace B. A.1234ORCID

Affiliation:

1. Institute of Structural and Molecular Biology

2. Birkbeck University of London

3. London WC1E 7HX

4. UK

5. School of Biological and Chemical Sciences

6. Queen Mary University of London

7. London E1 4NS

Abstract

This tutorial review discusses current methods and resources available for good practice data collection and analyses of circular dichroism spectroscopy, a widely-used method for examining the structures and conformational changes of proteins.

Funder

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Reference44 articles.

1. Circular Dichroism and the Conformational Analysis of Biomolecules , ed. G. D. Fasman , Plenum Press , New York , 1996

2. B. A. Wallace and R. W.Janes , in Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy , ed. B. A. Wallace and R. W. Janes , IOS Press , Amsterdam, The Netherlands , 1st edn, 2009 , ch. 1, pp. 1–18

3. S. C. Goodchild , K.Jayasundera and A.Rodger , in Biomolecular and Bioanalytical Techniques: Theory, Methodology and Applications , ed. V. Ramesh , Wiley , New Jersey, USA , 1st edn, 2019 , ch. 15, pp. 365–384

4. The role of circular dichroism spectroscopy in the era of integrative structural biology

5. Vibrational optical activity: From discovery and development to future challenges

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