Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems-Reference-Cited by-同舟云学术

Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems

Published:2021 Issue:27 Volume:11 Page:16285-16296
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Dhandapani Priyavathi¹²³⁴,Dondapati Srujan Kumar¹²³⁴,Zemella Anne¹²³⁴,Bräuer Dennis¹²³⁴,Wüstenhagen Doreen Anja¹²³⁴,Mergler Stefan⁵⁶⁷⁴,Kubick Stefan¹²³⁴⁸^ORCID

Affiliation:

1. Fraunhofer Institute of Cell Therapy and Immunology

2. Branch of Bioanalytics and Bioprocesses (IZI-BB)

3. Potsdam-Golm

4. Germany

5. Department of Ophthalmology

6. Charité – Universitätsmedizin Berlin

7. Berlin

8. Faculty of Health Sciences

Abstract

Carboxylesterase, the key enzyme to handle ester-based dyes, is synthesized in microsomes using eukaryotic cell-free protein synthesis platform and established as a viable calcium imaging tool to analyze native and cell-free synthesized ion channels.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/RA/D0RA08397F

Reference55 articles.

1. A comprehensive map of molecular drug targets

2. Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems

3. Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

4. Cell-Free Protein Synthesis: A Promising Option for Future Drug Development

5. Intraluminal calcium as a primary regulator of endoplasmic reticulum function

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Mathematical modeling of intracellular calcium in presence of receptor: a homeostatic model for endothelial cell;Biomechanics and Modeling in Mechanobiology;2022-10-11