A microfluidic device for the batch adsorption of a protein on adsorbent particles

Author:

Rho Hoon Suk1234ORCID,Hanke Alexander Thomas56784,Ottens Marcel56784,Gardeniers Han1234ORCID

Affiliation:

1. Mesoscale Chemical Systems Group

2. MESA+ Institute for Nanotechnology

3. University of Twente

4. The Netherlands

5. BioProcess Engineering group

6. Department of Biotechnology

7. Faculty of Applied Sciences

8. Delft University of Technology

Abstract

A microfluidic platform or “microfluidic batch adsorption device” is presented, which performs two sets of 9 parallel protein incubations with/without adsorbent particles to achieve an adsorption isotherm of a protein in a single experiment.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference47 articles.

1. J. D. Andrade , Surface and Interfacial Aspects of Biomedical Polymers: Volume 1 Surface Chemistry and Physics, Springer Science & Business Media, 2012

2. J.-C. Janson , Protein purification: principles, high resolution methods, and applications, John Wiley & Sons, 2012

3. Adsorption, desorption and re-adsorption of proteins on solid surfaces

4. R. K. Scopes , Protein purification: principles and practice, Springer Science & Business Media, 2013

5. M. L. Shuler and F.Kargi, Bioprocess engineering, Prentice Hall, New York, 2002

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