ELISA-based detection of fresh and frozen–thawed lamb meat: a promising analytical approach for meat authentication

Author:

Rahman C. K. Faslu1ORCID,Biswas Ashim Kumar1ORCID,Agrawal Ravikant12ORCID,Biswas Sanchay Kumar3,Bora Bedika1,Anand T. S.1,Ajay Argana4,Sen Arup Ratan1

Affiliation:

1. Division of Livestock Products Technology, India

2. Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

3. Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

4. Division of Livestock Production Management, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

Abstract

Biomarker based sELISA was developed for differentiation of chilled and frozen-thawed lamb meat for authentication. The guinea pig anti-SOD pAb and rabbit anti-SOD pAb were used as capture and detection Abs, respectively. The assay showed excellent sensitivity and selectivity.

Funder

Ministry of Food Processing Industries

ICAR-Indian Veterinary Research Institute

Publisher

Royal Society of Chemistry (RSC)

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