A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry-Reference-Cited by-同舟云学术

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A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry

Published:2014 Issue:5 Volume:139 Page:992-995
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Nunes-Miranda J. D.¹²³⁴⁵,Núñez Cristina⁶⁷⁸⁹¹⁰,Santos Hugo M.⁴⁵²³⁶,Vale G.⁶⁷⁸⁹¹⁰,Reboiro-Jato Miguel¹¹¹²¹³¹⁴¹⁵,Fdez-Riverola Florentino¹¹¹²¹³¹⁴¹⁵,Lodeiro Carlos⁶⁷⁸⁹¹⁰,Miró Manuel¹⁶¹⁷¹⁸¹⁹,Capelo J. L.⁶⁷⁸⁹¹⁰

Affiliation:

1. Department of Genetics and Biotechnology

2. University of Trás-os-Montes and Alto Douro

3. Vila Real, Portugal

4. Institute for Biotechnology and Bioengineering

5. Centre of Genomics and Biotechnology

6. REQUIMTE

7. Departamento de Química

8. Faculdade de Ciencias e Tecnologia

9. FCT

10. Universidade Nova de Lisboa

11. SING Group

12. Informatics Department

13. Higher Technical School of Computer Engineering

14. University of Vigo

15. Ourense, Spain

16. FI-TRACE Group

17. Department of Chemistry

18. University of the Balearic Islands

19. Palma de Mallorca, Spain

Abstract

A novel mesofluidic platform integrating on-chip probe ultrasonication for automated high-throughput shotgun proteomic assays.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AN/C3AN02178E

Reference48 articles.

1. Mass spectrometry and proteomics: hitting the mark

2. Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques

3. Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins

4. Influence of the Protein Staining in the Fast Ultrasonic Sample Treatment for Protein Identification through Peptide Mass Fingerprint and Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

5. Ultra Fast Trypsin Digestion of Proteins by High Intensity Focused Ultrasound

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