Hybrid mass spectrometry methods reveal lot-to-lot differences and delineate the effects of glycosylation on the tertiary structure of Herceptin®-Reference-Cited by-同舟云学术

Hybrid mass spectrometry methods reveal lot-to-lot differences and delineate the effects of glycosylation on the tertiary structure of Herceptin®

Published:2019 Issue:9 Volume:10 Page:2811-2820
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Upton Rosie¹²³⁴⁵^ORCID,Migas Lukasz G.¹²³⁴⁵^ORCID,Pacholarz Kamila J.¹²³⁴⁵^ORCID,Beniston Richard G.⁶⁷⁵,Estdale Sian⁶⁷⁵^ORCID,Firth David⁶⁷⁵^ORCID,Barran Perdita E.¹²³⁴⁵^ORCID

Affiliation:

1. Manchester Institute of Biotechnology

2. Michael Barber Centre for Collaborative Mass Spectrometry

3. University of Manchester

4. Manchester

5. UK

6. Covance Laboratories Ltd.

7. Harrogate

Abstract

To quantify the measurable structural heterogeneity of a biopharmaceutical product and the effect of glycosylation on this we systematically evaluate three lots of Herceptin®, two mAb standards and an intact 5 Fc-hinge fragment.

Funder

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/SC/C8SC05029E

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