Centrifugal micro-channel array droplet generation for highly parallel digital PCR-Reference-Cited by-同舟云学术

Centrifugal micro-channel array droplet generation for highly parallel digital PCR

Published:2017 Issue:2 Volume:17 Page:235-240
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Chen Zitian¹²³⁴⁵,Liao Peiyu¹²³⁴⁵,Zhang Fangli¹²³⁴⁵,Jiang Mengcheng¹²³⁴⁵,Zhu Yusen⁶⁷⁵⁸⁹,Huang Yanyi¹²³⁴⁵^ORCID

Affiliation:

1. Biodynamic Optical Imaging Center (BIOPIC)

2. Beijing Advanced Innovation Center for Genomics (ICG)

3. Peking-Tsinghua Center for Life Sciences

4. and College of Engineering

5. Peking University

6. Integrated Science Program

7. Yuanpei College

8. Beijing

9. China

Abstract

Aqueous solutions can be dispersed into picoliter droplets through a micro-channel array via centrifugation which produces monodispersed water-in-oil emulsion for digital PCR.

Funder

Ministry of Science and Technology of the People's Republic of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2017/LC/C6LC01305H

Reference48 articles.

1. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

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4. A new mathematical model for relative quantification in real-time RT-PCR

5. Quantification of mRNA using real-time RT-PCR

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