Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay

Author:

Zhang Yan12345,Hu Jinping12345,Zou Xiaoran12345,Ma Fei6789,Qiu Jian-Ge1011129,Zhang Chun-yang12345ORCID

Affiliation:

1. Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong

2. Key Laboratory of Molecular and Nano Probes

3. Ministry of Education

4. Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals

5. College of Chemistry

6. School of Chemistry and Chemical Engineering

7. Southeast University

8. Nanjing 211189

9. China

10. Academy of Medical Sciences

11. Zhengzhou University

12. Zhengzhou

Abstract

We develop a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification.

Funder

National Natural Science Foundation of China

Taishan Scholar Foundation of Shandong Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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