A relative quantitation method for measuring DNA methylation and hydroxymethylation using guanine as an internal standard

Author:

Németh Krisztina12,Mészáros Katalin3,Szabó Borbála4,Butz Henriett34,Arányi Tamás56,Szabó Pál T.2ORCID

Affiliation:

1. Institute of Chemistry, Eötvös Loránd University, Pázmány Péter stny. 1/A, H-1117 Budapest, Hungary

2. MS Metabolomics Research Group, Centre for Structural Study, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar tudósok krt. 2, H-1117 Budapest, Hungary

3. Hereditary Tumours Research Group, Eötvös Loránd Research Network, Semmelweis University, Szentkirályi u. 46, H-1088 Budapest, Hungary

4. Department of Laboratory Medicine, Semmelweis University, Bókay János u. 53-54, H-1089 Budapest, Hungary

5. Institute of Enzymology, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar tudósok krt. 2, H-1117 Budapest, Hungary

6. Department of Molecular Biology, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary

Abstract

A relative LC-MS/MS quantitation method for DNA methylation study, where in situ guanine was used as internal standard.

Funder

National Research, Development and Innovation Office

Hungarian Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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