On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode-Reference-Cited by-同舟云学术

On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

Published:2016 Issue:2 Volume:16 Page:269-281
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Georgi V.¹²³⁴⁵,Georgi L.⁶⁷⁸⁴,Blechert M.¹²³⁴,Bergmeister M.¹²³⁴,Zwanzig M.⁶⁷⁸⁴,Wüstenhagen D. A.⁵⁹¹⁰⁴,Bier F. F.⁵⁹¹⁰⁴,Jung E.¹²³⁴,Kubick S.⁵⁹¹⁰⁴

Affiliation:

1. Fraunhofer Institute for Reliability Microintegration

2. Department System Integration & Interconnection Technologies

3. Working Group Medical Microystems

4. Germany

5. Fraunhofer Institute for Cell Therapy and Immunology (IZI)

6. Technische Universität Berlin

7. Faculty Electrical Engineering Computer Science

8. Microperipheric Technologies

9. Branch Bioanalytics and Bioprocesses Potsdam-Golm (IZI-BB)

10. Potsdam

Abstract

The microfluidic device for cell-free protein synthesis enables a novel reaction mode with continuous in vitro transcription and translation in separate compartments combined by magnetic and microfluidic actuated mRNA transfer.

Funder

Bundesministerium für Bildung und Forschung

Fraunhofer-Gesellschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C5LC00700C

Reference50 articles.

1. Expression without boundaries: Cell-free protein synthesis in pharmaceutical research

2. The past, present and future of cell-free protein synthesis

3. Cell-free protein synthesis: Applications come of age

4. Developing cell-free biology for industrial applications

5. A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins

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