Novel application of pH-sensitive firefly luciferases as dual reporter genes for simultaneous ratiometric analysis of intracellular pH and gene expression/location-Reference-Cited by-同舟云学术

Novel application of pH-sensitive firefly luciferases as dual reporter genes for simultaneous ratiometric analysis of intracellular pH and gene expression/location

Published:2014 Issue:12 Volume:13 Page:1661-1670
ISSN:1474-905X
Container-title:Photochem. Photobiol. Sci.
language:en
Short-container-title:Photochem. Photobiol. Sci.

Author:

Gabriel Gabriele V. M.¹²³⁴⁵^ORCID,Viviani Vadim R.¹²³⁴⁵

Affiliation:

1. Laboratory of Biochemistry and Biotechnology of Bioluminescence

2. Department of Physics

3. Chemistry and Mathematics

4. Federal University of São Carlos (UFSCar)

5. Sorocaba, Brazil

Abstract

Firefly luciferase pH-sensitivity can be harnessed for ratiometric intracellular pH estimation, providing the first dual reporter system using a single luciferase gene.

Funder

SÃ£o Paulo Research Foundation

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Springer Science and Business Media LLC

Subject

Physical and Theoretical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/PP/C4PP00278D

Reference39 articles.

1. A new gastric-emptying mouse model based on in vivo non-invasive bioluminescence imaging

2. Beetle Luciferases: Colorful Lights on Biological Processes and Diseases