A novel analytical principle using AP site-mediated T7 RNA polymerase transcription regulation for sensing uracil-DNA glycosylase activity-Reference-Cited by-同舟云学术

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A novel analytical principle using AP site-mediated T7 RNA polymerase transcription regulation for sensing uracil-DNA glycosylase activity

Published:2020 Issue:12 Volume:145 Page:4321-4327
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Gao Weichen¹²³⁴,Xu Jin⁵⁶⁴,Lian Guowei¹²³⁴,Wang Xiaojun⁷⁸⁹⁴,Gong Xiaoqun¹²³⁴^ORCID,Zhou Dianming⁷⁸⁹⁴^ORCID,Chang Jin¹²³⁴^ORCID

Affiliation:

1. School of Life Sciences

2. Tianjin University and Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin)

3. Tianjin 300072

4. China

5. Tianjin Hospital

6. Tianjin 300211

7. Department of Toxicology

8. Tianjin Centers for Disease Control and Prevention

9. Tianjin 300011

Abstract

udgactivity could regulateT7 RNApolymerase transcription ability by the heteroduplex substrates with chemical modifications.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AN/D0AN00509F

Reference37 articles.

1. Genome maintenance mechanisms for preventing cancer

2. DNA MISMATCH REPAIR

3. Nucleotide Excision Repair in Mammalian Cells

4. Chemistry of Glycosylases and Endonucleases Involved in Base-Excision Repair

5. Formation, detection and repair of AP sites

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1. DNA repair-retarded isothermal CRISPR amplification for rapid, sensitive base excision repair enzyme assay;Analytica Chimica Acta;2024-07

2. Enzyme-Assisted Nucleic Acid Amplification in Molecular Diagnosis: A Review;Biosensors;2023-01-19

3. Target-activated T7 transcription circuit-mediated multiple cycling signal amplification for monitoring of flap endonuclease 1 activity in cancer cells;The Analyst;2023

4. Sensitive detection of abasic sites in double-stranded DNA based on the selective reaction of enzymes;Analytica Chimica Acta;2022-08

5. Recent advances in biosensor for DNA glycosylase activity detection;Talanta;2022-03

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