Exploiting orientation-selective DEER: determining molecular structure in systems containing Cu(ii) centres

Author:

Bowen Alice M.12345,Jones Michael W.1234,Lovett Janet E.12346,Gaule Thembanikosi G.7891011,McPherson Michael J.7891011,Dilworth Jonathan R.1234,Timmel Christiane R.1234,Harmer Jeffrey R.123412

Affiliation:

1. Centre for Advanced Electron Spin Resonance

2. University of Oxford

3. Oxford

4. UK

5. Institute of Physical and Theoretical Chemistry

6. SUPA

7. Astbury Centre for Structural Molecular Biology

8. Institute of Molecular and Cellular Biology

9. Faculty of Biological Sciences

10. University of Leeds

11. Leeds LS2 9JT

12. Centre for Advanced Imaging

Abstract

Analysis of orientation-selective DEER measurements using Cu(ii) centres in a series of molecules demonstrates its limits and capabilities in structure elucidation.

Funder

Engineering and Physical Sciences Research Council

Royal Society

Biotechnology and Biological Sciences Research Council

Australian Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Reference61 articles.

1. Dead-Time Free Measurement of Dipole–Dipole Interactions between Electron Spins

2. EPR distance measurements in deuterated proteins

3. Site-directed mutagenesis of colicin E1 provides specific attachment sites for spin labels whose spectra are sensitive to local conformation

4. DeerAnalysis2006—a comprehensive software package for analyzing pulsed ELDOR data

5. A. M. Bowen , C. E.Tait, C. R.Timmel and J. R.Harmer, in Structure and bonding: spin-labels and intrinsic paramagnetic centres in the biosciences: structural information from distance measurements, ed. C. R. Timmel and J. R. Harmer, SpringerLink, 2013, vol. 152

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