An effective urobilin clearance strategy based on paramagnetic beads facilitates microscale proteomic analysis of urine-Reference-Cited by-同舟云学术

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An effective urobilin clearance strategy based on paramagnetic beads facilitates microscale proteomic analysis of urine

Published:2024 Issue:13 Volume:149 Page:3625-3635
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhen Kemiao¹,Hou Wenhao¹,Bai Lu²,Wang Mingchao¹,Yue Zhan¹,Xu Zanxin¹,Xiong Deyun²,Gao Li²,Ying Wantao¹^ORCID

Affiliation:

1. State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, China

2. Department of Urology Surgery, The Second Affiliated Hospital of Guilin Medical University, Guilin, 541199, China

Abstract

We demonstrated that the combination of SP3 with precipitation achieved highly efficient urobilin removal, and enhanced the robustness of urine proteome analysis.

Funder

National Basic Research Program of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D4AN00312H

Reference63 articles.

1. Targeted proteomics identifies liquid-biopsy signatures for extracapsular prostate cancer

2. Human Urine Proteome: A Powerful Source for Clinical Research

3. Noninvasive urinary protein signatures associated with colorectal cancer diagnosis and metastasis

4. The Urine Proteome in Toxicology

5. Urine biopsy technologies: Cancer and beyond

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