A genetically encoded sensor with improved fluorescence intensity for opioid detection at cellular resolution-Reference-Cited by-同舟云学术

A genetically encoded sensor with improved fluorescence intensity for opioid detection at cellular resolution

Published:2021 Issue:81 Volume:57 Page:10560-10563
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Kroning Kayla E.¹²^ORCID,Li Mingcheng¹²,Petrescu D. Isabel¹³,Wang Wenjing¹²^ORCID

Affiliation:

1. Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA

2. Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA

3. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA

Abstract

We engineered an improved opioid sensor, called M-SPOTIT2, which is 11x brighter than our previously engineered M-SPOTIT1.1. Scale bar, 20 μm.

Funder

University of Michigan

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2021/CC/D1CC04524E

Reference16 articles.

1. Untangling the complexity of opioid receptor function

2. Molecular Mechanisms of Opioid Receptor-dependent Signaling and Behavior

3. Designing a Single Protein‐Chain Reporter for Opioid Detection at Cellular Resolution

4. Circularly permuted green fluorescent proteins engineered to sense Ca2+

5. Structural insights into µ-opioid receptor activation

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