Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses-Reference-Cited by-同舟云学术

Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses

Published:2018 Issue:21 Volume:10 Page:10241-10249
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Modi Souvik¹²³⁴⁵^ORCID,Higgs Nathalie F.¹²³⁴⁵^ORCID,Sheehan David¹²³⁴⁵,Griffin Lewis D.⁶⁷⁵^ORCID,Kittler Josef T.¹²³⁴⁵^ORCID

Affiliation:

1. Neuroscience

2. Physiology and Pharmacology

3. University College London. Gower Street

4. London

5. UK

6. Department of Computer Science

7. University College London

Abstract

An anti-GFP nanobody conjugated QD optical probe was applied to study single particle trackingin vitroandex vivo. This small, highly specific probe recognized GFP when expressed at the cell membrane and reported diffusion dynamics of the underlying target protein.

Funder

Medical Research Council

Biotechnology and Biological Sciences Research Council

FP7 Ideas: European Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2018/NR/C7NR09130C

Reference61 articles.

1. The Fluid Mosaic Model of the Structure of Cell Membranes