Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles-Reference-Cited by-同舟云学术

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Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

Published:2019 Issue:22 Volume:144 Page:6689-6697
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhao Han¹²³⁴,Yan Ying¹²³⁴,Chen Mingjian¹²³⁴,Hu Tingting¹²³⁴,Wu Kefeng¹²³⁴,Liu Haisheng¹²³⁴,Ma Changbei¹²³⁴^ORCID

Affiliation:

1. School of Life Sciences

2. Central South University

3. Changsha 410013

4. China

Abstract

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

Funder

National Natural Science Foundation of China

State Key Laboratory of Chemo/Biosensing and Chemometrics

Hunan University

Central South University

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AN/C9AN01659G

Reference49 articles.

1. Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme

2. XRCC1 Stimulates Human Polynucleotide Kinase Activity at Damaged DNA Termini and Accelerates DNA Single-Strand Break Repair

3. Assays for alkaline phosphatase activity: Progress and prospects

4. Highly sensitive detection of DNA phosphorylation by counting single nanoparticles

5. Enzyme action at 3' termini of ionizing radiation-induced DNA strand breaks.

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