A real-time LAMP-based dual-sample microfluidic chip for rapid and simultaneous detection of multiple waterborne pathogenic bacteria from coastal waters

Author:

Jin Jinglei12345,Duan Lijun12345,Fu Jiali12345,Chai Fangchao12345,Zhou Qianjin12345,Wang Yaohua6784,Shao Xinbin6784,Wang Lei9104,Yan Maocang6784,Su Xiurong12345,Zhang Yanjun11124,Pan Junhang11124,Chen Jiong12345ORCID

Affiliation:

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products

2. Ningbo University

3. Ningbo

4. China

5. School of Marine Sciences

6. Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource

7. Zhejiang Mariculture Research Institute

8. Wenzhou 325005

9. CapitalBio Corporation

10. Beijing 102206

11. Zhejiang Provincial Center for Disease Control and Prevention

12. Hangzhou 310009

Abstract

(1) A dual-sample microfluidic chip integrated LAMP method was developed to simultaneously detect 10 waterborne pathogenic bacteria within 35 min. (2) Its operations are in a highly automated format and it is suitable for on-site detection.

Funder

Natural Science Foundation of Ningbo

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

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