Stepwise conversion of the Cys<sub>6</sub>[4Fe–3S] to a Cys<sub>4</sub>[4Fe–4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase-Reference-Cited by-同舟云学术

Stepwise conversion of the Cys₆[4Fe–3S] to a Cys₄[4Fe–4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase

Published:2023 Issue:40 Volume:14 Page:11105-11120
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Schmidt Andrea¹,Kalms Jacqueline¹,Lorent Christian²^ORCID,Katz Sagie²,Frielingsdorf Stefan²^ORCID,Evans Rhiannon M.³^ORCID,Fritsch Johannes²,Siebert Elisabeth²,Teutloff Christian⁴,Armstrong Fraser A.³^ORCID,Zebger Ingo²^ORCID,Lenz Oliver²^ORCID,Scheerer Patrick¹^ORCID

Affiliation:

1. Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Medical Physics and Biophysics (CC2), Group Structural Biology of Cellular Signaling, Charitéplatz 1, 10117 Berlin, Germany

2. Institut für Chemie, Biophysical Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany

3. Department of Chemistry, University of Oxford, OX1 3QR Oxford, UK

4. Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany

Abstract

The [4Fe-3S] cluster of an O2-tolerant [NiFe] hydrogenase was transformed into different [4Fe-4S] clusters, and structural, spectroscopic and electrochemical analyses of the enzyme variants revealed an O2-tolerance mechanism at various levels.

Funder

Deutsche Forschungsgemeinschaft

Biotechnology and Biological Sciences Research Council

Einstein Stiftung Berlin

Horizon 2020 Framework Programme

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC03739H

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