A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity-Reference-Cited by-同舟云学术

A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity

Published:2023 Issue:36 Volume:14 Page:9892-9899
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Beriashvili David¹^ORCID,Yao Ru²^ORCID,D'Amico Francesca³^ORCID,Krafčíková Michaela¹^ORCID,Gurinov Andrei¹^ORCID,Safeer Adil¹,Cai Xinyi²,Mulder Monique P. C.³^ORCID,Liu Yangping²^ORCID,Folkers Gert E.¹,Baldus Marc¹^ORCID

Affiliation:

1. NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

2. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, P. R. China

3. Department of Cell and Chemical Biology, Leiden University Medical Center (LUMC), Einthovenweg 20, 2333 ZC, Leiden, The Netherlands

Abstract

We show that the polarising agent SNAPol-1 enters human cells yielding 800 MHz DNP solid-state NMR data of remarkable sensitivity & resolution thereby revealing structural heterogeneity of ubiquitin at atomic scale and with sub-cellular specificity.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

National Natural Science Foundation of China

H2020 Marie Skłodowska-Curie Actions

European Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC02117C

Reference61 articles.