A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities-Reference-Cited by-同舟云学术

A diffusion NMR method for the prediction of the weight-average molecular weight of globular proteins in aqueous media of different viscosities

Published:2019 Issue:2 Volume:11 Page:142-147
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Arrabal-Campos Francisco M.¹²³⁴⁵,Aguilera-Sáez Luis M.¹²³⁴⁵^ORCID,Fernández Ignacio¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemistry and Physics

2. Research Centre CIAIMBITAL

3. University of Almería

4. Almería

5. Spain

Abstract

We have introduced for the first time, a non-viscosity dependent universal calibration curve (UCC) for the successful prediction of the weight-average molecular weights of globular proteins in the range of 8.5–66.2 kDa with no dependence on the solvent viscosity.

Funder

Agencia de Innovación y Desarrollo de Andalucía

Ministerio de Ciencia e Innovación

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2019/AY/C8AY01817K

Reference31 articles.

1. Globular proteins at solid/liquid interfaces

2. Streptokinase is a flexible multi-domain protein

3. Detection of Insulin Aggregates with Pulsed-Field Gradient Nuclear Magnetic Resonance Spectroscopy

5. Characterization of Globular Protein Solutions by Dynamic Light Scattering, Electrophoretic Mobility, and Viscosity Measurements

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