Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells-Reference-Cited by-同舟云学术

Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells

Published:2021 Issue:36 Volume:12 Page:11955-11964
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Jones Chloe M.¹²^ORCID,Robkis D. Miklos¹²^ORCID,Blizzard Robert J.³,Munari Mika⁴,Venkatesh Yarra¹^ORCID,Mihaila Tiberiu S.¹^ORCID,Eddins Alex J.³,Mehl Ryan A.³^ORCID,Zagotta William N.⁴^ORCID,Gordon Sharona E.⁴^ORCID,Petersson E. James²^ORCID

Affiliation:

1. Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA, 19104, USA

2. Biochemistry and Molecular Biophysics Graduate Group, University of Pennsylvania, 3700 Hamilton Walk, Philadelphia, PA, 19104, USA

3. Department of Biochemistry and Biophysics, Oregon State University, 2011 Ag Life Sciences Building, Corvallis, Oregon, 97331, USA

4. Department of Physiology and Biophysics, University of Washington, 1705 NE Pacific St., Box 357290, Seattle, WA, 98195, USA

Abstract

Acridonylalanine (Acd) is photostable, with a high quantum yield and long fluorescence lifetime in water. An evolved tRNA synthetase (RS) enables genetic incorporation of Acd in mammalian cells and its use in fluorescence lifetime imaging microscopy.

Funder

National Institutes of Health

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D1SC01914G

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