High throughput mass spectrometry-based characterisation of Arabidopsis thaliana group H glycosyltransferases-Reference-Cited by-同舟云学术

High throughput mass spectrometry-based characterisation of Arabidopsis thaliana group H glycosyltransferases

Published:2018 Issue:53 Volume:8 Page:30080-30086
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Akere Aishat¹²³⁴^ORCID,Liu Qian⁵⁶⁷⁸⁹,Wu Shibo¹²³⁴,Hou Bingkai⁵⁶⁷⁸⁹,Yang Min¹²³⁴^ORCID

Affiliation:

1. The School of Pharmacy

2. University College London

3. London WC1N 1AX

4. UK

5. Key Laboratory of Plant Cell Engineering and Germplasm Innovation

6. Ministry of Education of China

7. School of Life Sciences

8. Shandong University

9. Jinan

Abstract

We cloned and characterised four group H glycosyltransferases by studying their substrate specificities and kinetics. Sequence alignment and site-directed mutagenesis studies showed that serine is a crucial residue for UDPGlcNAc and UDPGal activity.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/RA/C8RA03947J

Reference32 articles.

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