Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers-Reference-Cited by-同舟云学术

Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers

Published:2021 Issue:9 Volume:13 Page:1191-1203
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Flanagan Shane Patrick¹²³^ORCID,Fogel Ronen¹²³^ORCID,Edkins Adrienne Lesley⁴⁵²³^ORCID,Ho Lance St. John¹²³^ORCID,Limson Janice¹²³^ORCID

Affiliation:

1. Biotechnology Innovation Centre

2. Rhodes University

3. South Africa

4. Biomedical Biotechnology Research Unit (BioBRU)

5. Department of Biochemistry and Microbiology

Abstract

The nonspecific uptake of aptamers by dead cells is an often-overlooked factor during the study of aptamer binding to their targetsin vivo. Accounting for this can aid in the identification of aptamers with high affinity and specificity.

Funder

National Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D0AY01878C

Reference129 articles.

1. The Prioritization of Cancer Antigens: A National Cancer Institute Pilot Project for the Acceleration of Translational Research

2. The MUC1 SEA Module Is a Self-cleaving Domain

3. Autoproteolysis coupled to protein folding in the SEA domain of the membrane-bound MUC1 mucin