Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell-Reference-Cited by-同舟云学术

Ultrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell

Published:2021 Issue:3 Volume:12 Page:1001-1006
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Cong Yongzheng¹²³⁴,Motamedchaboki Khatereh⁵⁶⁴,Misal Santosh A.¹²³⁴^ORCID,Liang Yiran¹²³⁴,Guise Amanda J.⁷⁸⁴,Truong Thy¹²³⁴,Huguet Romain⁵⁶⁴,Plowey Edward D.⁷⁸⁴,Zhu Ying⁹¹⁰¹¹⁴^ORCID,Lopez-Ferrer Daniel⁵⁶⁴,Kelly Ryan T.¹²³⁴⁹^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry

2. Brigham Young University

3. Provo

4. USA

5. Thermo Fisher Scientific

6. San Jose

7. Biogen, Inc.

8. Cambridge

9. Environmental Molecular Sciences Laboratory

10. Pacific Northwest National Laboratory

11. Richland

Abstract

The combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS) and latest-generation mass spectrometry instrumentation provides dramatically improved single-cell proteome profiling.

Funder

National Cancer Institute

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC03636F

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