Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays

Author:

Lipp Clémentine1ORCID,Koebel Laure2,Loyon Romain3,Bolopion Aude2,Spehner Laurie3,Gauthier Michaël2ORCID,Borg Christophe3,Bertsch Arnaud1,Renaud Philippe1

Affiliation:

1. Laboratory of Microsystems LMIS4, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

2. Institut FEMTO-ST, Département AS2M, Univ. Bourgogne Franche-Comté, CNRS, Besançon, France

3. Unité RIGHT, UMR INSERM 1098, Établissement Français du Sang Bourgogne Franche-Comté, Besançon, France

Abstract

A novel microfluidic device capable of controlling the contact between single beads or cells is presented and its capabilities are demonstrated with an adhesion assays and specific interactions between T-cells and tumor cells.

Funder

Agence Nationale de la Recherche

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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