Derivatization-assisted enzyme-linked immunosorbent assay for identifying hallucinogenic mushrooms with enhanced sensitivity

Author:

Morita Izumi1ORCID,Kiguchi Yuki1,Oyama Hiroyuki1,Takeuchi Atsuko1,Tode Chisato1,Tanaka Rie2,Ogata Jun2,Kikura-Hanajiri Ruri2,Kobayashi Norihiro1

Affiliation:

1. Kobe Pharmaceutical University, 4-19-1, Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan

2. National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan

Abstract

Chemical derivatization into a larger molecule dramatically improved sensitivity of ELISA targeting psilocin, a major psychoactive compound in hallucinogenic mushrooms.

Funder

Ministry of Health, Labour and Welfare

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference48 articles.

1. Hallucinogenic mushrooms drug profile, accessed 22 June 2021, https://www.emcdda.europa.eu/publications/drug-profiles/hallucinogenic-mushrooms

2. Psilocybin – Summary of knowledge and new perspectives

3. P. P.Wieczorek , D.Witkowska , I.Jasicka-Misiak , A.Poliwoda , M.Oterman and K.Zielińska , in Studies in Natural Products Chemistry , Elsevier , Amsterdam , 2015 , vol. 46 , pp. 133–168

4. Immunochemical monitoring of psilocybin and psilocin to identify hallucinogenic mushrooms

5. Production and characterization of monoclonal antibodies against a novel 1α,25-dihydroxyvitamin D3-bovine serum albumin conjugate linked through the 11α-position

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