A DNA walker triggered isothermal amplification method based on freezing construction of AuNP probes and its application in ricin detection

Author:

Sun Xuan1ORCID,Wang Weiya2,Chai Yanyan3,Zheng Zhou1,Wang Yu2ORCID,Bi Jing1,Wang Qian1,Hu Yonggang3,Gao Zhixian2

Affiliation:

1. School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

2. Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin 300050, China

3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Abstract

A DNA walker triggered exponential amplification method based on freezing construction of AuNP probes is developed to detect ricin, showing a lower detection limit, satisfactory specificity, and excellent stability in practical application.

Funder

Key Research and Development Plan of Tianjin

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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