Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA-Reference-Cited by-同舟云学术

Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA

Author:

Meagher Robert J.¹²³⁴^ORCID,Priye Aashish¹²³⁴,Light Yooli K.¹⁵³⁴,Huang Cheng⁶⁷⁸⁴^ORCID,Wang Eryu⁶⁹¹⁰⁸⁴

Affiliation:

1. Sandia National Laboratories

2. Biotechnology and Bioengineering Department

3. Livermore

4. USA

5. Systems Biology Department

6. University of Texas Medical Branch

7. Department of Pathology

8. Galveston

9. Department of Microbiology and Immunology

10. 301 University Blvd

Abstract

Bumping the priming sites for RT-LAMP based on thermodynamic predictions can dramatically reduce non-specific background amplification.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

Reference31 articles.

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