Directly utilizing an endogenous gene to dissect regulatory elements in the biosynthetic gene cluster of nosiheptide-Reference-Cited by-同舟云学术

Directly utilizing an endogenous gene to dissect regulatory elements in the biosynthetic gene cluster of nosiheptide

Published:2014 Issue:72 Volume:50 Page:10430-10433
ISSN:1359-7345
Container-title:Chem. Commun.
language:en
Short-container-title:Chem. Commun.

Author:

Ma Min¹²³,Xue Yanjiu¹²³,Liu Weiying¹²³,Zhang Hong¹²³,Kong Lingyi¹²³,Wang Shuzhen¹²³,Chen Yijun¹²³

Affiliation:

1. State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology

2. China Pharmaceutical University

3. Nanjing, China

Abstract

Coupling the deletion of an endogenous gene and a non-integrative promoter-probe vector allowed reliable identification of biosynthetic promoters of nosiheptide.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2014/CC/C4CC04974H

Reference19 articles.

1. Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

2. Gene expression in Streptomyces: Construction and application of promoter-probe plasmid vectors in Streptomyces lividans

3. Chromogenic identification of promoters in Streptomyces lividans by using an ampC β-lactamase promoter-probe vector

4. Construction and application of a promoter-probe plasmid that allows chromogenic identification in Streptomyces lividans

5. Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator

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