Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless reader-Reference-Cited by-同舟云学术

Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless reader

Published:2022 Issue:8 Volume:22 Page:1531-1541
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Wang Yunxiang¹²,Chen Hong¹²,Wei Hongjuan¹²,Rong Zhen¹²^ORCID,Wang Shengqi¹²

Affiliation:

1. Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China

2. Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing 100850, P. R. China

Abstract

Wild-type SARS-CoV-2 or its D614G and N501Y mutations can be selectively amplified by tetra-primer ARMS-PCR and detected by two test lines on a single dual-color fluorescent test strip with a handheld wireless device.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D1LC01167G

Reference57 articles.

1. Genetic Variants of SARS-CoV-2—What Do They Mean?

2. Quasispecies Theory and the Behavior of RNA Viruses

3. SARS-CoV-2 variants and ending the COVID-19 pandemic