Rapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip

Author:

Zath Geoffrey K.12ORCID,Sperling Ralph A.34,Hoffman Carter W.125ORCID,Bikos Dimitri A.12ORCID,Abbasi Reha12ORCID,Abate Adam R.6ORCID,Weitz David A.37ORCID,Chang Connie B.125ORCID

Affiliation:

1. Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA

2. Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA

3. Department of Physics, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA

4. Fraunhofer Institute for Microengineering and Microsystems IMM, Mainz, Germany

5. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA

6. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, USA

7. Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA

Abstract

The Plate-Interfacing Parallel Encapsulation (PIPE) microfluidic chip generates drops of up to 96 different conditions in parallel, such as barcoded drop libraries, by interfacing individual drop makers with a standard 384-well microtiter plate.

Funder

Defense Advanced Research Projects Agency

National Institutes of Health

National Science Foundation

Materials Research Science and Engineering Center, Harvard University

Deutsche Forschungsgemeinschaft

Montana State University

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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