A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy

Author:

Nebuloni Federico12ORCID,Do Quyen B.345ORCID,Cook Peter R.2ORCID,Walsh Edmond J.1ORCID,Wade-Martins Richard345ORCID

Affiliation:

1. Osney Thermofluids Institute, Department of Engineering Science, University of Oxford, Osney Mead, Oxford OX2 0ES, UK

2. The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK

3. Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, UK

4. Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, UK

5. Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA

Abstract

In vivo, different neurons make directional connections; however, most in vitro models fail to capture such connectivity. We use microfluidics with fluid walls to study neuronal circuits in vitro in ways that fit into existing bio-medical workflows.

Funder

Agency for Science, Technology and Research

Aligning Science Across Parkinson's

Parkinson's UK

National Institute for Health Research

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

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