Spatially isolated reactions in a complex array: using magnetic beads to purify and quantify nucleic acids with digital and quantitative real-time PCR in thousands of parallel microwells-Reference-Cited by-同舟云学术

Spatially isolated reactions in a complex array: using magnetic beads to purify and quantify nucleic acids with digital and quantitative real-time PCR in thousands of parallel microwells

Published:2020 Issue:10 Volume:20 Page:1771-1779
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Henley W. Hampton¹²³⁴,Siegfried Nathan A.¹²³⁴,Ramsey J. Michael¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemistry

2. University of North Carolina at Chapel Hill

3. Chapel Hill

4. USA

5. Department of Biomedical Engineering

Abstract

Encoded beads carrying primer pairs for nucleic acid targets are used for sample preparation and multiplexed-in-space digital PCR quantification.

Funder

Defense Advanced Research Projects Agency

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/D0LC00069H

Reference44 articles.

1. A highly multiplexed broad pathogen detection assay for infectious disease diagnostics

2. Point-Counterpoint: Large Multiplex PCR Panels Should Be First-Line Tests for Detection of Respiratory and Intestinal Pathogens

3. Multiplex Molecular Panels for Diagnosis of Gastrointestinal Infection: Performance, Result Interpretation, and Cost-Effectiveness

4. Advances in multiplex nucleic acid diagnostics for blood-borne pathogens: promises and pitfalls

5. Microfluidic Devices for Bioapplications

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