A one-pot CRISPR-RCA strategy for ultrasensitive and specific detection of circRNA-Reference-Cited by-同舟云学术

A one-pot CRISPR-RCA strategy for ultrasensitive and specific detection of circRNA

Published:2024 Issue:20 Volume:16 Page:3256-3262
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Ke Xinxin¹,Liang Ajuan²,Chen Chuanxia³^ORCID,Hu Tao¹⁴^ORCID

Affiliation:

1. Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, China

2. Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Obstetrics and Gynecology Hospital, School of Medicine, Tongji University, Shanghai 201204, China

3. School of Materials Science and Engineering, University of Jinan, Jinan 250022, China

4. School of Basic Medical Sciences & Forensic Medicine, Hangzhou Medical College, Hangzhou 310000, China

Abstract

In this work, we introduce the application of the CRISPR/Cas12a system to establish a one-pot, rapid (30 minutes to 2 hours), specific and ultrasensitive circRNA detection strategy, termed RETA-CRISPR (Reverse Transcription-Rolling Circle Amplification (RT-RCA) with the CRISPR/Cas12a).

Funder

National Natural Science Foundation of China

Basic Public Welfare Research Program of Zhejiang Province

Zhejiang University

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AY/D4AY00693C

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