HIV pre-exposure prophylaxis adherence test using reverse transcription isothermal amplification inhibition assay

Author:

Zhang Jane Y.1ORCID,Zhang Yu2,Bender Andrew T.1ORCID,Sullivan Benjamin P.1ORCID,Olanrewaju Ayokunle O.1ORCID,Lillis Lorraine3,Boyle David3,Drain Paul K.456,Posner Jonathan D.125ORCID

Affiliation:

1. Department of Mechanical Engineering, University of Washington, Seattle, WA, USA

2. Department of Chemical Engineering, University of Washington, Seattle, WA, USA

3. PATH, Seattle, WA, USA

4. Department of Global Health, University of Washington, Seattle, WA, USA

5. Department of Medicine, University of Washington, Seattle, WA, USA

6. Department of Epidemiology, University of Washington, Seattle, WA, USA

Abstract

We report on a probabilistic isothermal nucleic acid amplification assay for measuring a common medication in HIV prevention and treatment. Expanded access to routine HIV drug adherence testing is a crucial tool in improving the HIV care continuum.

Funder

National Institute of Biomedical Imaging and Bioengineering

National Institute of Allergy and Infectious Diseases

Center for AIDS Research, University of Washington

M.J. Murdock Charitable Trust

University of Washington

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference58 articles.

1. WHO|HIV/AIDS , http://www.who.int/gho/hiv/en/ , (accessed 9 October 2019)

2. Statistics Overview|Statistics Center|HIV/AIDS|CDC , https://www.cdc.gov/hiv/statistics/overview/index.html , (accessed 9 October 2019)

3. R.Goldstein , PrEP prevents HIV — so why aren’t more people taking it? , https://www.health.harvard.edu/blog/prep-prevents-hiv-so-why-arent-more-people-taking-it-2019100417942 , (accessed 23 March 2020)

4. PrEP|HIV Basics|HIV/AIDS|CDC , https://www.cdc.gov/hiv/basics/prep.html , (accessed 11 March 2020)

5. C. S. C. for D. Control, P. last updated: January 16, and 2020, U.S. Statistics, https://www.hiv.gov/hiv-basics/overview/data-and-trends/statistics , (accessed 12 March 2020)

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