Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance-Reference-Cited by-同舟云学术

Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance

Published:2020 Issue:7 Volume:22 Page:2234-2243
ISSN:1463-9262
Container-title:Green Chemistry
language:en
Short-container-title:Green Chem.

Author:

Lenz Florian¹²³⁴,Zurek Paul¹²³⁴^ORCID,Umlauf Martina¹²³⁴,Tozakidis Iasson E. P.¹²³⁴,Jose Joachim¹²³⁴^ORCID

Affiliation:

1. Institute for Pharmaceutical and Medicinal Chemistry

2. PharmaCampus

3. 48149 Münster

4. Germany

Abstract

We evolved the thermophilic β-glucosidase CsBglA to 150% improvement of k_cat/K_M at 55 °C, exerting a specific activity of 504 U mg⁻¹ and a K_M-value of 37.1 mM, making it the bacterial β-glucosidase with highest activity known so far.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

Pollution,Environmental Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/GC/C9GC04166D

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