Design of molecular beacons: 3′ couple quenchers improve fluorogenic properties of a probe in real-time PCR assay-Reference-Cited by-同舟云学术

Design of molecular beacons: 3′ couple quenchers improve fluorogenic properties of a probe in real-time PCR assay

Published:2014 Issue:11 Volume:139 Page:2867-2872
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Ryazantsev Dmitry Y.¹²,Kvach Maksim V.³⁴,Tsybulsky Dmitry A.³⁴,Prokhorenko Igor A.¹²⁵⁶,Stepanova Irina A.¹²,Martynenko Yury V.³⁴,Gontarev Sergey V.⁷⁴,Shmanai Vadim V.³⁴,Zavriev Sergey K.¹²,Korshun Vladimir A.¹²⁵⁶

Affiliation:

1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

2. 117997 Moscow, Russia

3. Institute of Physical Organic Chemistry

4. 220072 Minsk, Belarus

5. Gause Institute of New Antibiotics

6. 119021 Moscow, Russia

7. Primetech LLC

Abstract

Fluorescent properties of molecular beacons with two dyes or two quenchers in real-time PCR are reported.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AN/C4AN00081A

Reference47 articles.

1. Molecular Beacons: Probes that Fluoresce upon Hybridization

2. Multicolor molecular beacons for allele discrimination

3. Molecular beacons

4. Molecular Engineering of DNA: Molecular Beacons

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