Fluorescent staining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by 4H-[1]-benzopyrano[4,3-b]thiophene-2-carboxylic acid hydrazide-Reference-Cited by-同舟云学术

Fluorescent staining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by 4H-[1]-benzopyrano[4,3-b]thiophene-2-carboxylic acid hydrazide

Published:2014 Issue:11 Volume:139 Page:2764-2773
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhu Zhongxin¹²³⁴⁵,Zhou Xuan¹²³⁴,Wang Yang⁶⁷³⁴,Chi Lisha¹²³⁴,Ruan Dandan¹²³⁴,Xuan Yuanhu¹²³⁴,Cong Weitao¹²³⁴⁵,Jin Litai¹²³⁴⁵

Affiliation:

1. School of Pharmaceutical Sciences

2. Zhejiang Provincial Key Laboratory of Biotechnology Pharmaceutical Engineering

3. Wenzhou Medical University

4. Wenzhou, China

5. Wenzhou Undersun Biotechnology Co., Ltd.

6. Institute of Neuroscience

7. Department of Histology and Embryology

Abstract

A sensitive, specific, economic and MS compatible staining method for gel-separated glycoproteins by using BH was described and demonstrated by 1-D and 2-D SDS-PAGE, deglycosylation, glycoprotein affinity enrichment and LC-MS/MS, respectively.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AN/C3AN02309E

Reference21 articles.

1. Glycosylation in Cellular Mechanisms of Health and Disease

2. A glycomic approach to the identification and characterization of glycoprotein function in cells transfected with glycosyltransferase genes

3. Intracellular Functions of N-Linked Glycans

4. Glycosylation, Immunity, and Autoimmunity

5. Electrophoretic methods for process monitoring and the quality assessment of recombinant glycoproteins

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