An enzyme-mediated bioorthogonal labeling method for genome-wide mapping of 5-hydroxymethyluracil-Reference-Cited by-同舟云学术

An enzyme-mediated bioorthogonal labeling method for genome-wide mapping of 5-hydroxymethyluracil

Published:2021 Issue:42 Volume:12 Page:14126-14132
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Ma Cheng-Jie¹,Li Lin²^ORCID,Shao Wen-Xuan¹,Ding Jiang-Hui¹,Cai Xiao-Li³,Lun Zhao-Rong³,Yuan Bi-Feng¹⁴^ORCID,Feng Yu-Qi¹⁴^ORCID

Affiliation:

1. Sauvage Center for Molecular Sciences, Department of Chemistry, Wuhan University, Wuhan 430072, China

2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, P. R. China

3. Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P. R. China

4. School of Public Health, Wuhan University, Wuhan 430071, China

Abstract

We developed an enzyme-mediated bioorthogonal labeling strategy for the enrichment and genome-wide mapping of 5hmU. With this strategy, we provided the first map of 5hmU in the whole Trypanosoma brucei genome.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D1SC03812E

Reference32 articles.

1. Detection, Structure and Function of Modified DNA Bases

2. Fuller-Rylenes: Cross-Dimensional Molecular Carbons

3. Nucleic Acid Modifications in Regulation of Gene Expression

4. The diverse roles of DNA methylation in mammalian development and disease

5. Dynamic DNA methylation: In the right place at the right time

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